Column efficiency {height equivalent of theoretical plate} (HETP) is column length divided by theoretical plate number, which depends on retention volume divided by baseline peak width. High volume means good resolution, so small HETP is good. HETP depends on sideways diffusion, longitudinal diffusion {band spread}, mass transfer from phase to phase, and flow rate, which depend on viscosity. Optimum flow rate keeps diffusion minimal. Equations {von Deemster equation} can relate these quantities.
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Date Modified: 2022.0224