High-speed spinning {centrifugation}| can separate substances by density or particle size. Solid pellet can form at tube end. Sucrose solutions or cesium chloride have a density gradient.
mass
Centrifugation can find molecule mass. Terminal-velocity sedimentation rate through medium depends directly on particle molecular weight. Machine reaches sedimentation rate when molecule cross-sectional size and viscosity balance centrifugal force. Sedimentation rate also depends on temperature and solvent.
polymer
Polymer sedimentation rate depends on volume divided by acceleration. In density gradient, centrifugal force balances polymer diffusion at equilibrium density.
Physical Sciences>Chemistry>Analytical Chemistry>Centrifugation
5-Chemistry-Analytical Chemistry-Centrifugation
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Date Modified: 2022.0224