Gene-expression technologies can use cell extracts from different tissues, same tissues under different conditions, or same tissues under same conditions, over time sequences {serial analysis of gene expression} (SAGE) {expressed RNA}. From RNA, two cell extracts from same tissues under same conditions can make first-strand cDNA labeled with fluorescent dye, one with Cy3 and one with Cy5. Purified labeled cDNA solution soaks slides at optimum temperatures for times. Robots measure slide-spot probe-DNA and labeled-cDNA hybridization.
Biological Sciences>Genetics>Recombinant DNA>Gene Expression
4-Genetics-Recombinant DNA-Gene Expression
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Date Modified: 2022.0224