Finding sites {spot-finding algorithm} can use three methods: find isolated peaks, align grid, or align centroid. To find isolated features or peaks, first shrink image to convolve it with feature model and then find isolated peaks. To align grid, first find fiducials and then fit rows and columns. To align centroid, first move off grid and find intensity-weighted centroid, if signal-to-noise feature ratio allows.
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Date Modified: 2022.0224