Fiducials {fiducial-finding algorithm} are marks or shapes every 300 pixels, for automatic spot finding and row and column counting, without using quantitation-control scheme. Fiducials must be easy to distinguish from other image features and artifacts. Spotting and shrinking cause non-linear distortions and determine fiducial frequency needed. Row and column drift must be less than half the distance, five or six pixels, between features.
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Date Modified: 2022.0224